Molecular Effect of Variants in Toll-like Receptor 4 Gene in Saudi Patients with Type 2 Diabetes Mellitus

Single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 4 (TLR4) gene have been documented type 2 diabetes mellitus (T2DM) and other diseases Saudi population. We investigated relationship between rs11536889, rs4986790, rs4986791 SNPs TLR4 T2DM population; 105 patients with healthy controls were analyzed. The was amplified through PCR, followed by restriction fragment length polymorphism analysis for Sanger sequencing rs11536889 rs4986790 SNPs. clinical biochemical characteristics associated (p < 0.05). control subjects Hardy–Weinberg equilibrium > Alleles rs4986791, heterozygous codominant, dominant models However, SNP not Logistic regression showed that high-density lipoprotein cholesterol (HDLc) levels 0.001). Analysis of variance waist = 0.0005) hip circumferences 0.002) SNPs, SBP 0.001), DBP 0.002), HDLc 0.003), subjects. also haplotype 0.001) but linkage disequilibrium. gene–gene interaction three studied according to generalized multifactor dimensionality reduction model 0.0001). Dendrogram graphical depletion revealed a moderate association T2DM. results suggest are genotypically allelically patients. Future functional studies recommended validate genetic roles these pathogenesis progression diseases.